After much anticipation, we (Thermo Scientific Pierce) just launched our ChIP Assay Kit. The Pierce Agarose ChIP Assay Kit and Reagents is an excellent tool for beginner and experience researchers performing ChIP assays. The kit uses/includes high grade formaldehyde crosslinker and our optimized reagents for subcellular fractionation to recover high quality chromatin. In addition, the chromatin is further prepared using high grade MNase digestion in order to produce more uniform chromatin fragments than are typically achieved using a sonicator. Following the chromatin preparation, ChIP grade, pre-blocked Protein A/G agarose is used to perform the chromatin immunoprecipitation step, followed by recovery of the target DNA fragment by elution and Proteinase K digestion of the proteins.
This is another product I’m excited about having helped launch on www.piercenet.com. My kit product shot should be on the page shortly.